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Objective:To analyze the distributions of ABO and RhD blood groups by analyzing the basic data of blood group detection among voluntary blood donors in Huainan in 2021, to provide data support for blood recruitment, clinical use of blood, and emergency guarantee of rare groups of blood.Methods:ABO and RhD blood groups of 24 484 voluntary blood donors eligible for blood donation in 2021 were detected using the Metis150-8 automatic blood group analyzer, manual saline method, antihuman globulin method, and manual polybrene test. The blood group results were statistically analyzed.Results:Among 24 484 voluntary blood donors in Huainan in 2021, A blood group accounted for the highest proportion (7 463 cases, 30.48%), followed by O blood group (7 444 cases, 30.40%) and B blood group (7 056 cases, 28.82%), and the last was AB blood group (2 521 cases, 10.30%). A total of 143 cases of RhD-negative blood were detected, and the negative frequency of RhD was 0.58% (143/24 484). Among the RhD-negative blood samples, 43 cases of type A, 41 cases of type B, 46 cases of type O, and 13 cases of type AB were RhD-negative, accounting for 30.07%, 28.67%, 32.17%, and 9.09%, respectively. There was no statistical difference in the detection rate of Rh-negative blood among different ABO blood groups in Huainan ( χ2 = 0.36, P = 0.948). The ABO blood group distribution of voluntary blood donors in Huainan in 2021 was not identical to those of voluntary blood donors in Guangzhou, Yueyang, Xinjiang Bozhou, Zhangzhou, and Liuzhou. The proportion of type A blood donors in Yueyang was higher than those in other regions. The proportions of type B blood donors in Huainan, Xinjiang Bozhou, and Zhangzhou were higher than those in other regions. The proportion of type O blood donors in Liuzhou was higher than those in other regions. The proportions of type AB blood donors in Huainan and Xinjiang Bozhou were higher than those in other regions. Conclusion:The distributions of ABO and RhD blood groups among voluntary blood donors in Huainan region have certain regional characteristics. Central blood banks and medical institutions should reasonably store and supply blood according to the blood collection from voluntary blood donations and the needs of clinical transfusion, to prevent the occurrence of situations such as blood expiration and waste.
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Objective@#To construct the key elements of mental care program for cancer patients based on Swanson′s caring theory.@*Methods@#Based on Swanson′s caring theory, using expert group discussion and Delphi expert inquiry, screening indicators to establish psychosocial care program for cancer patients.@*Results@#The effective recovery rates of the two rounds of expert inquiries were 100% (34/34) and 94.12% (32/34), respectively. The coefficient of authority of experts was 0.89, and the coefficient of coordination of expert opinions was 0.411. The final training system includes five first-level indicators, 17 second-level indicators and 89 third-level indicators.@*Conclusion@#The mental care program can provide reference for clinical practice.
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Objective:To construct the key elements of mental care program for cancer patients based on Swanson′s caring theory.Methods:Based on Swanson′s caring theory, using expert group discussion and Delphi expert inquiry, screening indicators to establish psychosocial care program for cancer patients.Results:The effective recovery rates of the two rounds of expert inquiries were 100% (34/34) and 94.12% (32/34), respectively. The coefficient of authority of experts was 0.89, and the coefficient of coordination of expert opinions was 0.411. The final training system includes five first-level indicators, 17 second-level indicators and 89 third-level indicators.Conclusion:The mental care program can provide reference for clinical practice.
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Objective@#To examine nurses′ experiences regarding the benefits and obstacles of using WeChat in health education for patients with PICC-line.@*Methods@#A semi-structured interview was conducted with 12 nurses who using WeChat to assist health education for patients with PICC-line. Data were analyzed based on Colaizzi′s phenomenological research method.@*Results@#Ten themes of three aspects were extracted: the benefits of using WeChat in health education include reduction in medical care consumption and costs, reduction in workload, facilitating improvement in the quality of care, promotion of the nurse–patient relationship, improvement in self-worth and professional knowledge; the obstacles of using WeChat in health education include perceived risk, disturbance to personal life, Sense of uncertainty; the advice of using WeChat in health education.@*Conclusions@#Exploring the experiences and obstacles of nurses regarding using WeChat in health education for patients with PICC-line can help improve the quality of health education and meanwhile provide the reference and basis for clinical health education.
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Objective To examine nurses′ experiences regarding the benefits and obstacles of using WeChat in health education for patients with PICC-line. Methods A semi-structured interview was conducted with 12 nurses who using WeChat to assist health education for patients with PICC-line. Data were analyzed based on Colaizzi′s phenomenological research method. Results Ten themes of three aspects were extracted: the benefits of using WeChat in health education include reduction in medical care consumption and costs, reduction in workload, facilitating improvement in the quality of care, promotion of the nurse–patient relationship, improvement in self-worth and professional knowledge; the obstacles of using WeChat in health education include perceived risk, disturbance to personal life, Sense of uncertainty; the advice of using WeChat in health education. Conclusions Exploring the experiences and obstacles of nurses regarding using WeChat in health education for patients with PICC-line can help improve the quality of health education and meanwhile provide the reference and basis for clinical health education.
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Objective To explore the effect of advance directives on hope level and coping style in cancer patients. Methods A total of 168 cancerous person were selected as the research sample according to conveniences sampling. They were divided into two groups according to whether they were set up or not advance directives. A total of 88 patients without advance directives (control group) was given full rescue, and a total of 80 patients (experimental group) was given medical care according to the patient's prior wishes. The scores of the Cancer Hope Scale and the Medical Coping Questionnaire were compared between the two groups. Results The score of the Cancer Hope Scale was (24.83 ± 2.59) points in experimental group, (30.09±3.36) points in control group. The score of the Medical Coping Questionnaire was (41.21±2.65) points in experimental group, (35.85±2.63) points in control group. There was significant difference between the two groups (t=11.861, 17.817, P <0.01). Conclusions There are many mental health problems in cancer patients. Patients with advance directives can face the disease with a more positive attitude.
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ObjectiveTo explore the treatment and economic burden of urine incontinence (UI) in the rural elderly,and provide evidences for preventive intervention.MethodsA cluster sampling was carried out in 743 people (aged 60 years and over) from 5 villages dominated by Yanliu and Dafengshang health centers,Jixian County,Tianjin from 2007 to 2008.The information about general state of health,treatment and economic burden of disease were analyzed. Results The prevalence of UI was 33.4％ (248 cases) among the 743 elderly people.Only 12 patients (4.8％) with UI ever visited community health-care service centers or hospitals for diagnosis and treatment,among whom only 1 case visited hospital within 1 year,8 cases during 1 to 5 years,and 3 cases over 5 years.And the method of treatment was all drug therapy.Among 248 cases with UI,there were 227 cases (91.5％) without any treatment,9 cases (3.6％) buying medicines by themselves.The direct cost for treatment of UI was 80-12000 yuan RMB,including 2 cases below 100 yuan RMB,2 cases 100-1000 yuan RMB,7 cases 1001-5000 yuan RMB and 1 case over 10000 yuan RMB. Conclusions Prevalence of UI is high while hospital-visiting rate is very low,and the financial burden of disease is serious,thus intervention measures for UI should be taken for the elderly people in rural areas of Jixian County,Tianjin.
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We expressed and prepared the recombinant fusion protein sTNFRII-gAD consisted of soluble TNF receptor II and the globular domain of adiponectin by Adenovirus Vector System in mammalian BHK21c022 cells. First we used the adenovirus vector containing EGFP gene (rAd5-EGFP) to infect BHK21c022 cells at different MOI (from 0 to 1 000), and then evaluated their transduction efficiency and cytotoxicity. Similarly, we constructed the replication-deficient adenovirus type 5-sTNFRII-gAD (rAd5-sTNFRII-gAD). We collected the supernatants for Western blotting to determine the optimal MOI by comparing the expression levels of sTNFRII-gAD fusion protein, 48 h after the BHK21c022 cells were infected by rAd5-sTNFRII-gAD at different MOIs (from 0 to 1 000). Then, we chose rAd5-sTNFRII-gAD at MOI 100 to infect five bottles of BHK21c022 cells in 100 mL of serum-free chemically defined media 100 mL, harvested the supernatant every 48 h for 6 times, and condense and purify sTNFRII-gAD fusion protein by ammonium sulfate salt-out and size-exclusion chromatography, respectively. Finally, we analyzed anti-TNFalpha activity of sTNFRII-gAD fusion protein on L929 cells in vitro. The results showed that the number of BHK21c022 cells expressing EGFP protein was increased significantly with the increase of MOI. However, some cells died at MOI of 1 000 while there was no significant cytotoxicity at MOI from 0 to 100. Western blotting analysis showed that the more adenoviruses, the higher expression of sTNFRII-gAD fusion protein in the supernatant with the highest expression at MOI 1 000. We successfully obtained about 11 mg bioactive and purified sTNFRII-gAD fusion protein at last. The in vitro assay demonstrated that the sTNFRII-gAD fusion protein was potent to antagonize TNFalpha's cytotoxicity to L929 cells. Put together, we established a recombinant adenovirus vector/BHK21 cell expression system, characteristic of the efficient serum-free culture and easy scaling-up.
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Animals , Humans , Adenoviridae , Genetics , Metabolism , Adiponectin , Genetics , Cell Line , Genetic Vectors , Genetics , Green Fluorescent Proteins , Genetics , Metabolism , Receptors, Tumor Necrosis Factor, Type II , Genetics , Recombinant Fusion Proteins , GeneticsABSTRACT
To study the function of lipoprotein lipase (LPL)with Asn291 Ser and Lys312insC compound mutation in LPL gene knockout heterozygous (LPL~(+/-)) mice. The results showed that triglycerides, free fatty acids, blood glucose and weight of LPL~(+/-) mice were higher than those of c57 mice(P<0. 05). The expressions of LPL Mrna and LPL protein of LPL~(+/-) group were lower than those of c57 group(P<0.05). The injection of Asn291Ser +Lys312insC protein caused little change of the lipid mass and LPL activity,but the injection of normal LPL protein induced obvious decrease of lipid mass and increase of the LPL activity.
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Objective To explore the relationship of chronic diseases and the history of gestation and delivery with urinary incontinence (UI) among elderly people of rural areas, so as to lay a scientific foundation for formulating strategies of prevention and treatment for UI. Methods A cross-sectional study was carried out in two towns of Jixian county, Tianjin. A total of 743 people aged 60 years and over were selected with cluster sampling, and all information were collected with a standardized structured questionnaire by face-to-face interview. The relationships of chronic diseases and the history of gestation and delivery with UI were analyzed with univariate analysis and multivariate Logistic regression method. Results Prevalence of UI was 33. 4% among the people aged 60 years and over in two towns of Jixian, and it was higher in females than in males (43. 2% vs. 22.8%, χ~2= 34.70, P<0.0001). Logistic regression analysis revealed that the chronic respiratory diseases, prostate disease, neurological diseases or motor system diseases were all associated with UI in men, and the elderly men who suffered from prostate disease and neurological diseases had the highest risk of UI (OR=11. 47, OR=11. 76). Constipation, chronic respiratory diseases, diabetes mellitus and motor system diseases were all associated with UI in women, and the elderly women with chronic respiratory diseases had the highest risk of UI (OR = 4. 84). Elderly women who had more gravidity (OR = 1. 03), more parity (OR= 1.02), perineal laceration (OR = 1.72) and wound infection (OR= 1. 65) during delivery had higher prevalence of UI. Conclusions Prevalence of UI is higher among the elderly people in rural areas of Jixian county, and several chronic diseases and gestation and delivery history in women are all associated with UI in the elderly. For the prevention of UI, the elderly should actively look for treating their chronic diseases and the medical workers should strengthen health education or health care for pregnant women.
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Objective To investigate the effect of psychological intervention on level of hope and quality of life in the breast cancer patients.Methods Ninety five breast cancer patients were randomized into intervention group and control group for an eight-week observation.Herth hope index(HHI)and Quality of life core questionnaire for European tumor patients(QLQ-C30)were used to observe the effect of psychological intervention on level of hope and quality of life in the breast cancer patients.Results Hope scores in total and in three subcategories were higher in intervention group((40.43±4.25),(13.28±1.61),(13.62±1.70),(13.53±1.52))than that in control group((37.82±4.49),(12.29±1.89),(12.90±1.67),(12.63±1.51))with statistically significant differences(P＜0.05).Scores for general quality of life,body function and role function were higher in intervention group((82.27±15.11),(75.89±12.24),(72.34±22.86))than that in control group((65.82±14.16),(68.30±16.42),(53.06±27.36))with statistically significant differences(P＜0.05).Scores for fatigue,pain,insomnia and loss of appetite were lower in intervention group((39.48±14.62),(25.18±16.98),(29.79±24.31),(18.44±21.77))than that in control group((49.43±17.58),(36.74±19.54),(46.26±28.72),(40.82±22.84))with statistically significant differences(P＜0.01).Conclusion-Psychological intervention could improve the level of hope and quality of life in the breast cancer patients.
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Diabetic rat model was induced by high fat diet combined with streptozotocin (STZ). After the model was established, blood samples were taken from jugular veins to examine plasma adrenocorticotropic hormone (ACTH) and corticosterone, and hypothalamus and pituitary were removed for real-time PCR. There were no significant differences in basal plasma ACTH and corticosterone level among control, obese, and obese diabetic rats (P=0.07). The corticosterone rhythm in obese and obese diabetic rats was impaired. Hypothalamus glucocorticoid receptors (GR) mRNA expressions yielded similar results in the groups, but 11β-HSD1 mRNA expression in obese diabetic rats was up-regulated ( vs control rats, P<0.05 ). The expressions of GR and 11β-HSD1 in pituitary of obese diabetic and obese rats were significantly down-regulated (both P<0.05). In the obese diabetic rats, the impaired glucocorticoid negative feedback was partly due to down-regulation of 11 β-HSD1 and GR expressions in pituitary.
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ObjectiveTo understand the prevalence of urinary incontinence (UI) and its epidemiological characteristics in rural elderly people and to lay a scientific foundation for formulating the strategies oI prevention and treatment for UI. MethodsA cross-sectional study was carried out in two townships of Jixian County, Tianjin. People aged 60 years and over were selected with cluster sampling, and the prevalence of UI and its epidemiological characteristics were collected with a standardized structured questionnaire by face-to-face interview. ResultsPrevalence of UI was 33.4% among the people aged 60 years and over in two townships, and it was increased with ageing.The elderly women had a higher prevalence of UI than the elderly man (43.2% vs. 22.8%, P<0.0001). The prevalences of UI in groups of 60-, 65-, 70-, 75-, 80-, 85-95 years old were 28.6%,32.1%,34.1%,35.5%,47.8%and 30.0%, respectively, and increased with ageing (X2for trend=2.19,P=0.029). Mixed UI (MUI) was the most common in different types of UI, and the constitution ratios of stress UI (SUI), urge UI (UUI), MUI and other type of UI were 27.4%, 1.2%, 60.9%,10.5%, respectively. The prevalence of MUI was the highest among the four types of UI in each age group. In all age groups, the prevalences of SUI and UUI were the highest in 60～ age group, and the prevalence of MUI was the highest in 80 ～ 95 age group. ConclusionsPrevalence of UI is high among the elderly people in rural areas of Jixian County, Tianjin.
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Objective To observe the effect of herbal recipe Zhiganqing capsule(ZGQ capsule) in treating non-alcoholic fatty liver disease(NAFLD).Methods One hundred and twenty NAFLD patients were divided into two groups randomly.On the basis of diet control and increasing exercise,the treating group was treated with ZGQ capsule,and the control group was treated with Dongbao Gantai pill.The effect and side effects were observed after 4 months.Results The curing rate and total effective rate of treatment group and control group were 51.67%,93.33% and 13.34%,53.33% respectively,the difference between the two groups was significant(P